Unit 3.13: Beer-Lambert Law

Select the correct wavelength for maximum absorbance based on absorbance vs. wavelength graphs.

Use the absorption spectrum below to determine the wavelength a spectrophotometer should be set to determine the concentration of chlorophyll A.

absorption spectrum of chlorophyll

Image credit: BMG LABTECH – Absorbance

Use absorbance vs. concentration graphs to find A or c.

If ε = 1.2 L/mol·cm and b = 1.0 cm, what is A for a 0.20 M solution?

Recognize that the slope of A vs. c graphs equals εb.

Given a slope of 0.80 in an A vs. c graph with b = 1.0 cm, calculate ε.

Use A = εbc to calculate concentration, given path length and molar absorptivity.

A solution has A = 0.60, ε = 200 L/mol·cm, and b = 1.0 cm. What is its concentration?

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